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KMID : 0624620080410020153
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BMB Reports
2008 Volume.41 No. 2 p.153 ~ p.157
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Increased expression of the F1Fo ATP synthase in response to iron in heart mitochondria
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Kim Mi-Sun
Kim Jin-Sun
Cheon Choong-Ill
Cho Dae-Ho
Park Jong-Hoon
Kim Keun-Il
Lee Kyo-Young
Song Eun-Sook
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Abstract
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The objective of the present study was to identify mitochondrial components associated with the damage caused by iron to the rat heart. Decreased cell viability was assessed by increased presence of lactate dehydrogenase (LDH) in serum. To assess the functional integrity of mitochondria, Reactive Oxygen Species (ROS), the Respiratory Control Ratio (RCR), ATP and chelatable iron content were measured in the heart. Chelatable iron increased 15-fold in the mitochondria and ROS increased by 59%. Deterioration of mitochondrial function in the presence of iron was demonstrated by low RCR (46% decrease) and low ATP content (96% decrease). Using two dimensional gel electrophoresis (2DE), we identified alterations in 21 mitochondrial proteins triggered by iron overload. Significantly, expression of the &agr;, &bgr;, and d subunits of F1Fo ATP synthase increased along with the loss of ATP. This suggests that the F1Fo ATP synthase participates in iron metabolism. [BMB reports 2008; 41(2): 153-157]
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KEYWORD
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F1Fo ATP synthase, Heart mitochondria, Iron overload, Proteomics
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